Antinociceptive Effect of BPC-157 in The Formalin-induced Pain Model
Sang Yoong Park1, Chan Jong Chung1,Hae-Kyu Kim2, Rushin Maria Dass3
Department of Anesthesiology
and Pain Medicine, School of Medicine, Dong-A University, Busan, Republic of
Korea1
Department of Anesthesiology
and Pain Medicine, School of Medicine, Pusan National University, Busan,
Republic of Korea2
Department of Anesthesiology
and Critical Care, Kuala Lumpur General Hospital, Kuala Lumpur, Malaysia3
Background: BPC-157 is a stable gastric pentadecapeptide that effective in trials for wound healing capability, increased collagen and new blood vessels formation, decreased myeloperoxidase activity and inflammatory cell influx, including healing of muscle and tendon. BPC-157 also has undoubtedly a positive effect on muscle healing that may be provided partly by the regeneration of the damaged intramuscular nerve branches. There are no studies about the effect of BPC-157 on the pain transmission to the high level like central sensitization. This study was aimed to investigate the effect of BPC-157 on the central sensitization of pain from formalin test.
Methods: Male Sprague-Dawley rats (250~300g) were included in this study. All the experimental animals were randomly divided into Control (n=6), Morphine 5mg/kg (n=6) and BPC 3 groups (n=6). BPC group was randomly subdivided according to dosage of BPC-157 (n=6 in each groups). Formalin test was done as follows. For induction of pain, 50 μL of 5% formalin solution was applied to the hind paw. Pain behavior was quantified by periodically counting the number of flinches of the injected paw after injection. The number of flinches was counted for 1 min periods at 1 and 10 min and at 10 min intervals from 10 and 60 min. Morphine 5mg/kg, BPC-157 10, 20, 40 μg/kg in BPC group and normal saline (all same volume) in Control group were administered intraperitoneally 60 minutes before the formalin injection.
Results: Intraperitoneal administration of BPC 157 decreased dose-dependently the sum of the number of flinches during phase1, but not during phase 2 in the formalin test.
Conclusions: These findings indicated that BPC-157 was effective against a facilitated pain evoked by formalin injection at the peripheral level but not at the central level. Thus, the BPC-157 may be useful in the management of acute peripheral pain.
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Boban Blagaic A, Turcic P, Blagaic V, Dubovecak M, Jelovac N, Zemba M, et al. Gastric pentadecapeptide BPC 157 counteracts morphine-induced analgesia in mice. J Physiol Pharmacol. 2009; 60: 177-81.
